Fig. 2

Inhibition of ZIKV entry to clathrin-depleted cells. (a) Confocal analysis of ZIKV localization 10 min p.i. in Vero cells. vØ – ZIKV-infected, non-transfected cells; v + si – ZIKV-infected, clathrin-specific siRNA transfected cells; v + sc – ZIKV-infected, scrambled siRNA transfected cells; v + sh – ZIKV-infected, sham transfected cells; mock – mock-infected, non-transfected cells. ZIKV envelope protein is visualized in green and nuclei are shown in blue. Scale bar = 10 μm. (b) Co-localization between ZIKV envelope and clathrin presented as Manders’ coefficient M2 for control and clathrin-depleted Vero cells inoculated with ZIKV. The data is presented as mean ± SD. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001. (c) Western blot analysis of the efficiency of siRNA-dependent clathrin silencing (clathrin expression in Vero cells compared to GAPDH expression in these cells). M – BlueStar prestained protein marker; Ø – normal non-transfected Vero cells