Fig. 11From: Zika virus: mapping and reprogramming the entryInfluence of NH4Cl on ZIKV adhesion to the host cells. Flow cytometry analysis of viral adhesion to cells in the presence of NH4Cl was carried out. Vero cells pre-treated with 50 mM NH4Cl were overlaid with ZIKV stock and following 2 h incubation at 4 °C, they were fixed and ZIKV was immunostained with Alexa Fluor 488. Graph shows median fluorescence normalized to control, which corresponds to the proportion of ZIKV-positive cells in total cells population. vØ – inhibitor-untreated cells overlaid with ZIKV; NH4Cl –NH4Cl-treated cells overlaid with ZIKV; mock – mock-overlaid inhibitor-untreated cells. The data is presented as mean ± SD. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001Back to article page