Fig. 1

Co-localization between ZIKV and clathrin-dependent endocytosis markers. Confocal images of Vero cells showing co-localization of ZIKV envelope protein and clathrin (upper) or EEA1 (lower) at different time points p.i. Respective time points are indicated at the top of each column. Control denotes mock-infected cells, stained with anti-ZIKV envelope antibodies and rabbit isotype antibodies (control for staining of cellular proteins). The virus is visualized in green, clathrin and EEA1 are shown in red, and nuclei are presented in blue. Scale bar = 10 μm. Co-localization parameters: r – Pearson’s coefficient; M2 - Manders’ coefficient M2 (the virus overlapping with clathrin/EEA1)