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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Transcriptional positive cofactor 4 promotes breast cancer proliferation and metastasis through c-Myc mediated Warburg effect

Fig. 5

PC4 promotes glycolysis by regualting c-Myc transcription. a GSEA comparing the gene sets of Myc targets in PC4high (n = 78) and PC4low (n = 77) breast cancer patients. Data were obtained from GSE9893. NES means normalized enrichment score. b Protein levels of c-Myc were detected by western blotting in MDA-MB-231 and MCF-7 cells after PC4 knockdown. c mRNA levels of c-Myc were detected by qPCR in MDA-MB-231 and MCF-7 cells after PC4 knockdown. d, e, f and g After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, the ECAR was measured. h, i and j After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, glucose uptake, the production of lactate and ATP was measured. k After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, the protein levels of c-Myc, GLUT1, LDHA, E-cadherin, and Vimentin were detected. l After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, cell migration capacity was measured using the wound healing assay. m Statistical analysis of the data derived from l. Experiments were repeated three times independently. n After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, cell invasive capacity was detected using the transwell assay. o Statistical analysis of the data derived from (n). Experiments were repeated three times independently. p After overexpression of c-Myc in PC4 knockdown MDA-MB-231 cells, cell viability was measured by CCK-8 assay. q ChIP-PCR analysis for the PC4 occupancy on C-MYC promoters in MDA-MB-231 cells. All data indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

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