Fig. 4
Glycolysis is critically involved in the oncogenic functional of PC4. a GSEA comparing the gene sets of glycolysis in PC4high (n = 78) and PC4low (n = 77) breast cancer patients. Data was obtained from GSE9893. NES means normalized enrichment score. b, c and d Glucose uptake, the production of lactate and ATP were determined in PC4 knockdown MDA-MB-231 cells and control cells. e, f, g and h The extracellular acidification rate (ECAR) was determined in PC4 knockdown MDA-MB-231 cells and control cells. i Glycolysis markers (GLUT1 and LDHA) were detected by western blotting in MDA-MB-231 and MCF-7 cells after PC4 knockdown. j Cell migration capacity was measured using the wound healing assay after 2-DG treatment in PC4 overexpression MDA-MB-231 cells. k The specific plasmid was used to overexpress of PC4 and the protein level of PC4 was examined by western blot. l Statistical analysis of the data derived from (j). Experiments were repeated three times independently. m Cell invasive capacity was detected using the transwell assay in control cells, PC4 overexpression cells and 2-DG treated PC4 overexpression MDA-MB-231 cells. n Statistical analysis of the data derived from m. Experiments were repeated three times independently. o Cell viability was measured by CCK-8 assay in control cells, PC4 overexpression cells and 2-DG treated PC4 overexpression MDA-MB-231 cells. All data indicate the mean ± SD, *p < 0.05, **p < 0.01