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Table 2 Primer sequences for quantitative RT PCR

From: Estrogen regulation of cardiac cAMP-L-type Ca2+ channel pathway modulates sex differences in basal contraction and responses to β2AR-mediated stress in left ventricular apical myocytes

Gene name

 

Primer sequence (5′ → 3′)

Amplicon length [bp]

Forward

Reverse

 

Pde3a

NC_000072.6 Reference GRCm38.p4 C57BL/6J

TTTAACGCCAAGGTAAACGATG

TGATGTCAGCCAGCTTTATACA

97

Pde3b

NC_000073.6 Reference GRCm38.p4 C57BL/6J

GAAAAAGTGCCTGTGATCAGAC

TCTGTTCTCGGGAAATACAAGG

130

Pde4a

NC_000075.6 Reference GRCm38.p4 C57BL/6J

GCTGACCTGAAGACTATGGTAG

GATACGGTCAGAGTAGTTGTCC

81

Pde4b

NC_000070.6 Reference GRCm38.p4 C57BL/6J

CAGGAAAATGGTGATTGACATGGTGTTGG

CGAAGAACCTGTATCCGGTCAGTATAG

 

Pde4d

NC_000079.6 Reference GRCm38.p4 C57BL/6J

CCAGAATCTGACCAAAAAGCAA

GATCCTGTCGGAGTAGTTATCC

175

Atp2a2

NC_000071.6 Reference GRCm38.p4 C57BL/6J

TTCTGCTTATCTTGGTAGCCAA

CTTTCTGTCCTGTCGATACACT

125

Sln

NC_000075.6 Reference GRCm38.p4 C57BL/6J

ATGGAGAGGTCTACTCAGGAGCTG

ACCTCACGAGGAGCCACATAAGG

82

Adrb2

NC_000084.6 Reference GRCm38.p4 C57BL/6J

CACAAAGCCCTCAAGACTTTAG

CCTGATAACGTGCACGATATTG

93

Ryr2

NC_000079.6 Reference GRCm38.p4 C57BL/6J

CCATTGAGAACAAGTACATGCC

AATTCGTTGTTCATCATGAGCC

112

Plb

NC_000076.6 Reference GRCm38.p4 C57BL/6J

TACCTCACTCGCTCGGCTATCAG

CACAATGATGCAGATCAGCAGCAG

132

Cacna1c

NC_000072.6 Reference GRCm38.p4 C57BL/6J

GAGACAATGTGTGGAATATGCC

GTAGGTAGAGTTGACCACGTAC

103

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Cell Communication and Signaling

ISSN: 1478-811X