Fig. 5

eNOS knockdown and p38 inhibition alterations on endothelial resistance: a HMVECs were exposed to siRNA for 72 h (siControl or sieNOS). 30 min prior to Poly I:C exposure (10 μg/mL), cells were treated with SB203580 (SB, 10 μmol/L) or vehicle control (DMSO < 0.1%). Trans-endothelial electrical resistance (TEER) was determined by measuring resistance across the monolayer at 1 h intervals for a total of 24 h across the respective groups and normalized to the resistance 1 h prior to agonist treatment. b Total area under the curve (AUC) for changes in resistance (increase = positive, decrease = negative) relative to baseline for all data points collected. c AUC for time intervals of 0 to 8 h (left), 9 to 16 h (center) and 17 to 24 h (right) (n = 6 replicates per group). * = p < 0.05 between compared groups, † = p < 0.05 between group compared to respective siControl group, NS = non-significant