Fig. 2

eNOS knockdown induced changes in endothelial monolayer resistance: a HMVECs were exposed to siRNA for 72 h (siControl or sieNOS) and then treated with Poly I:C (10 μg/mL). Trans-endothelial electrical resistance (TEER) was determined by measuring resistance across the monolayer at 1 h intervals for a total of 24 h across the respective groups and normalized to the resistance 1 h prior to agonist treatment. b Total area under the curve (AUC) for changes in resistance. AUC was determined by aggregate change in resistance (increase = positive, decrease = negative) relative to baseline for all data points collected. c AUC for specific time intervals of 0 to 8 h (left), 9 to 16 h (center) and 17 to 24 h (right) (n = 4 replicates per group). * = p < 0.05 between compared groups, † = p < 0.05 between group compared to respective siControl group, NS = non-significant