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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Targeting p21-activated kinase 1 inhibits growth and metastasis via Raf1/MEK1/ERK signaling in esophageal squamous cell carcinoma cells

Fig. 3

Silencing PAK1 decreases the migratory and invasive abilities of ESCC cells. a Overexpression of PAK1 dramatically promoted the migratory ability of ESCC cells in a wound-healing assay. Scale bars, 200 μm. Representative images (left) and summary bar chart (right) of cells that migrated through a membrane (b) or invaded through a Matrigel-coated membrane (c). Column and error bar represents mean ± SD (n = 6 per group). Scale bars, 100 μm. *, P < 0.05, **, P < 0.01 and ***, P < 0.001 vs Vector group; P values were obtained by one-way ANOVA with post-hoc intergroup comparison with the Tukey’s test. d PAK1 deletion dramatically reduced the migratory ability of ESCC cells in a wound-healing assay. Silencing PAK1 resulted in a decrease in the migratory (e) and invasive (f) abilities of ESCC cells as determined by Transwell analysis. Representative images (left) and summary bar chart (right) are shown. Column and error bar represents mean ± SD (n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001 compared with shNC, one-way ANOVA, post-hoc intergroup comparisons, Tukey’s text. g Western blot analysis of MMP-2 and MMP-9 expression in PAK1-overexpresing and -silenced ESCC cells. Actin served as loading control

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