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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: DRAM1 regulates autophagy and cell proliferation via inhibition of the phosphoinositide 3-kinase-Akt-mTOR-ribosomal protein S6 pathway

Fig. 2

DRAM1 inhibits the mTORC1 signalling. a HEK293T cells were transfected with FLAG empty vector or FLAG-DRAM1 for 48 h, and the protein levels of p-mTOR (S2448), mTOR, p-p70S6K (T389), p70S6K, p-4EBP1, 4EBP1, p-rpS6 (S235/236, S240/244), rpS6, FLAG and β-actin were detected with immunoblotting. b, c and d Quantitative analysis of p-rpS6 (S240/244), p-p70S6K and p-4EBP1 in HEK293T cells transfected with FLAG-DRAM1. Data represent mean ± SEM of combined data from three independent experiments. e HEK293T cells were transfected with FLAG empty vector or FLAG-DRAM1 for 48 h and the protein levels of p-p53 (S15), p53, FLAG and β-actin were detected with immunoblotting. *p < 0.05 vs control

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Cell Communication and Signaling

ISSN: 1478-811X