Fig. 1
From: Autocrine motility factor promotes endometrial cancer progression by targeting GPER-1
AMF induces proliferation in an AMFR-independent manner in EC cells. a. Ishikawa and SPEC-2 cells were stably transfected with plasmid containing AMFR-specific shRNA (shAMFR-1 or shAMFR-2) or control plasmid (mock). Cells were then analyzed by qRT-PCR. b. Left, immunoblot analysis for AMFR and β-actin protein expression; Right, quantification of AMFR expression. C and D. RTCA and transwell assays for cell migration (c) and invasion (d). Cells were seeded onto the upper surfaces of chambers without (c) or with Matrigel coating (d) and analyzed after 30 h of incubation. Left, Cell index values were quantitated and are expressed as the mean ± SD from three independent experiments; right, Photographs depict the migration or invasion of EC cells (*P < 0.05; **P < 0.01, and ***P < 0.001 compared with the control cells). e. Growth curve by RTCA assay. Cells were seeded at a low density (2000 per well) and grown for 100 h (points, mean of triplicate determinations; bars, SD)