Fig. 4

GFPT2 is an immediate-early gene product expressed in mesenchymal NSCLC cells. a Mesenchymal A549 cells upregulate and maintain GFPT2 mRNA transcripts over a 96 h time course, while GFPT1 expression remains relatively unchanged. b Immunoblot analysis demonstrates an increase in GFPT2 protein expression that corresponds with protein markers of mesenchymal transition. c & d TNF/TGF-stimulated 3D NSCLC lines upregulate GFPT2 mRNA and protein expression. a & c RT-qPCR analysis represents three independent experiments performed in triplicate; mRNA levels were calculated relative to HPRT mRNA expression. Mean + SD are shown * p = < 0.05, ** = < 0.01, and < 0.005. Immunoblots were analyzed for GAPDH as a loading control (b) and (d). Densitometic analysis of immunoblots was used to quantitate fold change of GFPT2 relative to GAPDH