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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Regulation of platelet-activating factor-induced interleukin-8 expression by protein tyrosine phosphatase 1B

Fig. 7

PTP1B increases PAF-stimulated SFK activation in iMo-DCs. a and b siCTRL-iMoDCs or siPTP1B-iMoDCs were collected on day 7 and stimulated for indicated times with 10 nM PAF. Reaction was stopped on ice and cells were collected and lysed. Whole cell lysates were loaded onto SDS-PAGE, transferred to nitrocellulose membrane and blotted overnight with antibodies (Abs) recognizing vinculin, Src and a pTyr419 SFK or b non-phosphorylated form of Tyr530 of Src (non-Tyr530). The blots were stripped between re-blotting with indicated antibodies. Representative blots are shown for each experimental condition and compilations of at least 4 independent experiments are presented as mean ± S.E.M indices of normalized ratios of a pTyr419 SFK or b non-Tyr530 Src levels calculated as described in Methods. Significance was established with paired two-way ANOVA with Sidak post-test: * < 0.05, PTP1B vs control cells

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