Fig. 10

SFKs modulate PAF-induced Akt phosphorylation in a similar manner to PTP1B. iMo-DCs were collected on day 7, incubated for 20 min with 10 μM PTP1B inhibitor, 100 nM PP2, alone or in combination, or with their vehicles. Cells were stimulated with 10 nM PAF for indicated times. Reaction was stopped on ice and cells were collected and lysed. Lysates were separated on SDS-PAGE gels and Western blots were performed with anti-vinculin, anti-Ser473 Akt or anti-Akt Abs. Control and PP2-treated cells are shown on two separate blots. The blots were stripped between re-blotting with indicated antibodies. Representative blots are shown for each condition and compilation of experiments is presented as mean ± S.E.M of normalized ratios of pSer473 Akt levels calculated as described in Methods for 4 experiments. Significance was established with paired two-way ANOVA with Sidak post-test: *p < 0.05, **p < 0,01 vs control cells