Fig. 1From: Obesogenic high-fat diet heightens aerobic glycolysis through hyperactivation of oncogenic KRASHFD hyperactivates oncogenic KRAS to elevate aerobic glycolysis. a Diagram of mouse treatment. TM, tamoxifen; CD (n = 8), carbohydrate-rich diet; HFD (n = 8), high-fat diet. b-c Western blot analysis and quantification of the expression levels of HKII in fElasCreERT and KrasG12D/+ with the indicated treatment. Three independent experiments from three different mice per group were performed. d-e Western blot analysis and quantification of the expression levels of LDHA in fElasCreERT and KrasG12D/+ with the indicated treatment. Three independent experiments from three different mice per group were performed. f-g RAS activity assay followed by Western blot analysis and quantification of the RAS-GTP levels in mice harboring fElasCreERT and KrasG12D/+ under both CD and HFD for 10 weeks. Total RAS was used as a loading control. Three independent experiments from three different mice per group were performed. h Representative IHC staining for p-ERK (upper panel) and Alcian blue staining for acidic mucins (lower panel) as indicated. Magnifications: 200x. (n = 6 mice per group). i Statistical analysis of p-ERK (n = 6) as represented in Fig. 1h (upper panel). j Statistical analysis of the Alcian Blue staining (n = 6) as represented in Fig. 1h (lower panel). Data are mean ± SD. p values are determined by the Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001Back to article page