Fig. 7

HDAC3 inhibition induces CCA cell apoptosis by decreasing cMYC. a HuCCT1 cells were treated with 10 μM RGFP966 for 24 h and then endogenous proteins were visualized by Western blotting. b HDAC3-knockdown HuCCT1 cells and their counterparts were lysated and then endogenous proteins were visualized by Western blotting. c The OCR of cells were detected at different time points after they treated with 10 μM RGFP966 for 24 h. d HDAC3 knockdown HuCCT1 cells and control counterparts were injected at the left and right sides of the same mice to induce xenograft tumor in nude mice. The xenograft tumor sizes were measured. e The pyruvate levels of xenograft tumor were measured. f The protein levels in the xenograft were detected (left) and quantified (right). Scale bars, 100 μm. Data represent the Mean ± SEM, n ≥ 3. *p < 0.05, **p < 0.01, NS not significant