Fig. 4
Hypoxia signaling response is attenuated in MTA1 knockout cells and restored when cells are provided with MTA1 exosomes. MCF7 (a) and MDA-MB-231 (b) wildtype and MTA1 knockout (KO) cells were transfected with the HRE reporter plasmid. Cells were treated with 150 and 300 μM CoCl2 for 18 h. Cells were assayed for luciferase activity. (c) MDA-MB-231 wildtype and MTA1 knockout were transfected with the HRE reporter plasmid and incubated in normoxic (~ 20% O2) or hypoxic (1% O2) conditions for 6 h. MCF7 (d) and MDA-MB-231 (e) wildtype and MTA1 knockout cells were pre-incubated with 15 μg of exosomes from MTA1 overexpressing MCF7 and MDA-MB-231 cells, respectively and treated with 100 μM CoCl2 for 18 h. (f) MDA-MB-231 wildtype and MTA1 knockout cells were pre-incubated with 30 or 60 μg of exosomes from MTA1 overexpressing MDA-MB-231 cells and incubated in normoxic (20% O2) or hypoxic (1% O2) conditions for 6 h. All experiments were conducted in triplicate, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Two-way ANOVA