Skip to main content

Advertisement

Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells

Fig. 5

Oroxylin A inhibition of LDH-A led to blockade of aerobic glycolysis in HSCs. LX2 cells were treated with oroxylin A and/or galloflavin at indicated concentrations, or transfected with LDH-A siRNA for 24 h. a Measurements of glucose uptake. b Measurements of glucose consumption indicated by GOD activity. c Measurements of intracellular lactate levels. d Real-time PCR analyses of mRNA expression of HK2, PFK1 and PKM2. e Western blot analyses of protein expression of HK2, PFK1 and PKM2 with quantification. f Measurements of intracellular HK2 enzyme activity. g Measurements of intracellular PFK1 enzyme activity. h Measurements of intracellular PKM2 enzyme activity. i Real-time PCR analyses of mRNA expression of LDH-A. j Western blot analyses of protein expression of LDH-A with quantification. k Real-time PCR analyses of mRNA expression of HK2, PFK1 and PKM2. For statistical significance of panels a-h: *p < 0.05 vs. control, **p < 0.01 vs. control, ***p < 0.001 vs. control. For statistical significance of panels i-k: *p < 0.05 vs. control siRNA, **p < 0.01 vs. control siRNA, ***p < 0.001 vs. control siRNA

Back to article page