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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells

Fig. 2

Oroxylin A blocks aerobic glycolysis in HSCs. LX2 cells were treated with oroxylin A at indicated concentrations for 24 h. a Measurements of glucose uptake. b Measurements of glucose consumption indicated by GOD activity. c Measurements of intracellular lactate levels. d Measurements of ECAR. e Real-time PCR analyses of mRNA expression of HK2, PFK1 and PKM2. f Western blot analyses of protein expression of HK2, PFK1 and PKM2 with quantification. g Measurements of intracellular enzyme activities of HK2, PFK1 and PKM2. For statistical significance of this figure: *p < 0.05 vs. control, **p < 0.01 vs. control

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