Fig. 5
From: Helicobacter pylori-controlled c-Abl localization promotes cell migration and limits apoptosis
c-Abl downregulation reduced apoptosis. (a) AGS cells were left untreated or infected with Hp at the indicated MOIs and time periods. Percent apoptosis was calculated by analyzing combined Annexin single-positive and Annexin/propidium iodide-double positive cells. (b, c, d) Stable AGS cells transfected with control (shCtr) or c-Abl (shAbl) shRNA were left untreated or infected with Hp wt or a ∆cagA mutant at a MOI 20 for 48 h. Cell survival was determined using a MTT assay. (b) Equal protein amounts were subjected to human early apoptosis kit measurements and active caspase-8 (c) or caspase-9 (d) are shown