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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Helicobacter pylori-controlled c-Abl localization promotes cell migration and limits apoptosis

Fig. 2

PKC induces pAblT735 phosphorylation. (a) AGS cells were cotransfected with pSGT-Ablwt and si control, siTTK or siPKC and were left untreated or infected with Hp for 6 h. Lysates were analyzed for pAblT735, TTK, PKC and Abl. (b) AGS cells were left untreated, infected with Hp or stimulated with 100 nM PMA. Lysates were analyzed for pPKC, PKC and GAPDH. (c) pSGT-Ablwt-transfected AGS cells were treated with a PKA inhibitor (PKI) as a negative control or the PKC inhibitors Gö6983 and BIM. Where indicated, cells were infected with Hp and analyzed for pAblT735, Abl, pPKC, PKC and GAPDH. (d) Recombinant c-Abl (rAbl) and PKCα/β/γ (rPKC) were co-incubated with rottlerin (Rottl) or BIM. pAblT735, Abl and PKC proteins were detected

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