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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Low dose radiation regulates BRAF-induced thyroid cellular dysfunction and transformation

Fig. 5

LDR reduces activation of JAK2/STAT3 and downregulates miR-330-5p levels to reduce cellular transformation. a Phosphorylation status of JAK1, JAK2, STAT3, STAT5 was observed by western blot analysis in 850-5C and BCPAP thyroid cancer cells. b, c qPCR analysis for miR-330-5p and thyroid specific gene (PAX8, NIS and TG) levels in STAT3 overexpressing BCPAP thyroid cancer cells after LDR treatment. d Immunocytochemistry for NIS expression in STAT3 overexpressing BCPAP cells after LDR treatment. e, f Soft agar colonies and sphere formation was determined in BCPAP cells in same conditions as shown in D. Scale bar = 100 and 50μm. β-actin was used as a control for normalization. *p < 0.05, **p < 0.001, and ***p < 0.0001

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