Fig. 4
From: Low dose radiation regulates BRAF-induced thyroid cellular dysfunction and transformation
miR-330-5p targets PAX8 and is downregulated by LDR. PAX8 suppressed thyroid carcinogenesis after LDR exposure. a Venn diagram showing the PAX8 targeting miRNAs predicted by miRNA prediction tools as mentioned in panels. b Determination of PAX8 mRNA after anti-miR-144-3p and anti-miR-330-5p treatment in 850-5C thyroid cancer cells. c, d qPCR analysis of miR-144-3p and miR-330-5p levels in BRAFV600E transformed N-thy ori-3-1, BRAF-mutated 850-5C and BCPAP thyroid cancer cells after fractionated (0.01Gyx10) and single dose (0.1Gy) of LDR respectively. e, f miR-144-3p and miR-330-5p mimics were treated in LDR-exposed 850-5C and BCPAP cells and PAX8 levels were detected by western blot and qPCR analysis respectively. g Immunofluorescence of PAX8 levels in LDR-treated BCPAP thyroid cancer cells after miR-144-3p and miR-330-5p mimics transfection. h FACS analysis for PAX8 expression in LDR-treated BCPAP cells after miR-330-5p transfection. i, j TG, NIS mRNA levels and soft agar colony formation was analyzed in LDR-treated BCPAP thyroid cancer cells after miR-330-5p mimetics transfection. k TSHR levels were detected by ELISA assay in cell supernatants of LDR-treated BCPAP thyroid cancer cells in similar conditions as used in H. β-actin was used as a control for normalization. *p < 0.05, **p < 0.001, and ***p < 0.0001