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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Low dose radiation regulates BRAF-induced thyroid cellular dysfunction and transformation

Fig. 2

LDR blocks cellular transformation through upregulating iodine-metabolizing gene expression in BRAF-transformed thyroid cells. a Soft agar colony formation in BRAFV600E transformed N-thy ori-3-1 cells after fractionated (0.01Gy× 10) and single dose (0.1Gy) of LDR. b Quantification of tumor sphere formation in 850-5C and BCPAP thyroid cancer cells after fractionated (0.01Gy × 10) and single dose (0.1Gy) of LDR. c Relative mRNA expression of thyroid iodine metabolizing genes in BRAFV600E transformed N-thy ori-3-1 cells after fractionated (0.01Gy × 10) dose of radiation. d Relative mRNA expression of thyroid iodine metabolizing genes in 850-5C and BCPAP cells after fractionated (0.01Gyx10) and single dose (0.1Gy) of LDR. e Flow cytometry analysis of PAX8 positive cells in BRAFV600E transformed N-thy ori-3-1 cells after fractionated (0.01Gyx10) dose of radiation. f Immunofluorescence for NIS and PAX8 expression levels in 850-5C and BCPAP thyroid cancer cells after fractionated (0.01Gyx10) and single dose (0.1Gy) of LDR. g A non-radioactive iodine assay was performed in BCPAP thyroid cancer cells after fractionated (0.01Gyx10) and single dose (0.1Gy) of LDR. β-actin was used as a control for normalization. Scale bar = 50 μm; *p < 0.05, **p < 0.001, and ***p < 0.0001

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