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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Low dose radiation regulates BRAF-induced thyroid cellular dysfunction and transformation

Fig. 1

BRAF is highly expressed in thyroid cancer. a Analysis of BRAF expression in thyroid cancer among various cancer subtypes through publically available GENT database tool. b Evaluation of BRAF mutation frequency in papillary and follicular thyroid carcinoma using TCGA database. c Relative BRAF mRNA expression across normal follicular thyroid (N-thy ori-3-1), BRAF wild-type (BHP 10–3) and BRAF mutated-type (SNU-80, 850-5C, SNU-790, BCPAP) thyroid cancer cell lines. d ELISA assay for determination of TSHR levels in thyroid cells. e, f NIS and PAX8 mRNA expression in several thyroid cell lines respectively. g Immunohistochemistry for PAX8 expression in normal and PTC tumors as indicated in panels. h Co-relation studies of BRAF and PAX8 mRNA expression using TCGA database. PAX8 was negatively co-related with BRAF expression. i, j Soft agar colony and tumor sphere formation assay in BRAFV600E mutated normal N-thy ori-3-1 transformed thyroid cells. Each experiment represents the mean of at least two independent set of experiments. β-actin was used as a control for normalization. Scale bar = 100 μm; *p < 0.05, **p < 0.001, and ***p < 0.0001

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