Fig. 4

Effects of Cmk2 on both calcium sensitivity and CDRE-lacZ activity are dependent on its catalytic activity. (a), cells of the wild-type BY4741 and the cmk2 mutant carrying the pHAC111 vector, pHAC111-CMK2 or pHAC111-CMK2M were grown at 30 °C in liquid SD-LEU medium overnight, serially diluted by 10 times and spotted on YPD plates with or without 0.6 M CaCl₂, respectively. Plates were incubated for 2 to 3 days at 30 °C. (b), Galactosidase activities of 4xCDRE-lacZ in the wild-type (WT) BY4741and the cmk2 mutant, carrying the pHAC111 vector, pHAC111-CMK2 or pHAC111-CMK2M as indicated. They were grown at 30 °C in liquid SD-LEU medium overnight before they were inoculated to, and grown in, YPD medium to log phase. Cells were further grown in the absence or presence of 0.2 M CaCl₂ for 2 h before they were collected for protein extraction and lacZ activity assay. Symbols # and * show statistically significant differences (P < 0.05) between the wild type and each of other strains in the absence or presence of 0.2 M CaCl₂, respectively. Values were means of six independent experiments