Fig. 1
Pharmacological inhibition by memantine and genetic downregulation of Kv1.3 channels promote cell death of acute lymphoblastic leukemia cells. a The graph shows the dose response relationship for memantine of isolated Kv1.3 currents from Jurkat (JE6–1) cells. Each data point represents the mean ± SD of 5–7 cells from n = 3 independent experiments. b The membrane potential of untreated and memantine-treated Jurkat cells was determined by current clamp. KCl treatment served as a positive control; the mean + SD was calculated from 5 to 6 cells of n = 3 independent experiments. c Jurkat cells were treated with the indicated concentrations of memantine for 72 h and cell death was determined with propidium iodide (PI) staining and flow cytometry. Data gives the mean + SD percentage of PI+ cells from n = 5 independent experiments. d Jurkat cells were infected with lentivirus harboring shRNA against Kv1.3 channels (Sh-Kv1.3 (1) and Sh-Kv1.3 (2)) or scrambled sequence (Sh-scr). SYTOX staining was performed at day 7. Percentage and mean of SYTOX+ cells is shown for n = 6 independent experiments