Fig. 6

Effects of Cori treatment on AMPK/GSK3β signaling pathway in HepG2 cells. a HepG2 cells were exposed to concentration of Cori (30 μM) for three time points (1, 3 or 6 h), and AMPK and GSK3β phosphorylation were determined by Western blotting analysis. b Cells were exposed to CC (Compound C: an inhibitor of AMPK, 3 μM) for 18 h and then treated with Cori (30 μM) for 6 h. Effects of Cori on AMPK, GSK3β, ACC and LKB1 phosphorylation and Nrf2 protein expression. Moreover, (c) cells were exposed to SB (SB216763: an inhibitor of GSK3β, 100 nM) for 18 h and then treated with Cori (30 μM) for 6 h. Effects of Cori on GSK3β phosphorylation and Nrf2 protein expression. Additionally, (d) cells were exposed to CC (3 μM) for 1 h and then treated with Cori (30 μM) for 1 h, and then exposed to APAP (15 mM) for 24 h, cell viability was determined by CCK8 assay. The relative density of protein was performed by densitometric analysis; β-actin was acted as an internal control. Similar results were obtained from three independent experiments. All results were expressed as means ± SEM of three independent experiments. ^#p < 0.05, ^##p < 0.01 vs the Control group; ^**p < 0.01 vs the APAP group; ^&&p < 0.01 vs the Cori group