Fig. 5

Effects of Cori treatment on Nrf2 signaling pathway in Nrf2 ^−/− HepG2 cells. a WT and Nrf2^−/− HepG2 cells were extracted from total protein for detecting Western blotting analysis. b WT and Nrf2 ^−/− HepG2 cells were treated with and without Cori (30 μM) for 1 h, and then exposed to APAP (15 mM) for 24 h, cell viability was determined by CCK8 assay. c HepG2 WT and Nrf2 ^−/− cells were cultured with or without Cori (30 μM) for 6 h, and then immunoblotting was performed to measure Nrf2, GCLC, GCLM, NQO1 and HO-1. The relative density of protein was performed by densitometric analysis; β-actin was acted as an internal control. Similar results were obtained from three independent experiments. All results were expressed as means ± SEM of three independent experiments. ^##p < 0.01 vs the Control group; ^&&p < 0.01 vs the Cori group; ^**p < 0.01 vs the APAP group