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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Heme oxygenase 1 facilitates cell proliferation via the B-Raf-ERK signaling pathway in melanoma

Fig. 6

HO-1 regulates the cell cycle through B-Raf-ERK signaling. a Immunohistochemical staining for p-ERK in human melanoma tissue. Right panels are magnification from boxed fields in left panels. b Protein levels of ERK and p-ERK in HO-1-overexpressing, HO-1-knockdown and (c) HO-1−/− A375 cells. d Fluorescence analysis of B-Raf expression in HO-1-overexpressing cells and HO-1−/− cells . Scale bar: 10 μm. e HO-1−/− cells were transfected with Flag-B-Raf or the PCMV-Flag control as indicated. Forty-eight hours later, the ERK and p-ERK expression was analyzed by Western blotting. f Western blot analyses showing that B-Raf can activate p-ERK and that treatment with 20 μM HO-1 inhibitor (ZnPPIX) for 12 h can rescue the p-ERK expression of B-Raf. g The cells were pretreated with 20 μM PD98059 (ERK inhibitor) for 18 h after being transfected with B-Raf vector. The lysates were processed to measure p-ERK, ERK, cyclin E and CDK2. h HO-1 overexpression in cells treated with 20 μM PD98059 could significantly rescue the cell cycle protein expression changes induced by HO-1. i A carton showed HO-1 promotes proliferation by increasing CDK2 and cyclin E expression via B-RAF/ERK signaling

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