Fig. 4

HO-1-induced modulations in cell cycle-related proteins. a, b HO-1-overexpressing (a) and HO-1^−/− (b) A375 cells underwent cell cycle analysis by flow cytometry (left). Statistical analysis of the cell populations (%) at different stages of the cell cycle (right). c, d HO-1-overexpressing (c) and HO-1^−/− (d) A375 cells underwent RT-PCR and Western blot analysis of CDK2 and cyclin E. e A375 cells with or without CRISPR/Cas9 HO-1 knockout were subcutaneously injected into female SCID mice, q-PCR was performed for cell cycle regulator gene analysis at 45 days after injection. f The protein level of HO-1, B-Raf, cyclin E and CDK2 were evaluated by Western blotting in A375 cells treated with UV at 0, 25, 50, 100 KJ/m² after 12 h. g The protein level of HO-1, B-Raf, cyclin E and CDK2 were measured in A375 cells treated with 40 μM H₂O₂ for 6 h. *P < 0.05; **P < 0.01 by the t-test