Fig. 2
From: Heme oxygenase 1 facilitates cell proliferation via the B-Raf-ERK signaling pathway in melanoma
HO-1 promotes cell proliferation in A375 cells. a The overexpression efficiency of HO-1 in A375 cells were confirmed by Western blotting. Cell viability was evaluated by the CCK-8 assay. b Representative colony formation of A375 cells with HO-1 overexpression. The colony number in each well was determined and statistically analyzed with three independent experiments (n = 3). c Immunofluorescence staining of F-actin using phalloidin-fluorescein isothiocyanate in scramble and overexpression of HO-1 cells. Scale bar: 10 μm. d Representative tumor images were taken from the HO-1 overexpression group and control group. The tumor size (mm3) was measured every 5 day for 40 days after injection in both groups. e The tumor weight was recorded at the end of the experiment (Day 40) (n = 5). *P < 0.05; **P < 0.01 by the t-test