Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Blockade of ARHGAP11A reverses malignant progress via inactivating Rac1B in hepatocellular carcinoma

Fig. 5

ARHGAP11A directly interacts with Rac1B to exert its roles in HCC. Columns, mean (n = 3, in triplicate); bars, SD. *, P < 0.05 versus NC. ns, no significance. a Changes in mRNA expression of Rac1B following ARHGAP11A knockdown in HCC cells. b Changes in protein level of Rac1B in ARHGAP11A-knockdown HCC cells or NC. c MMP3 protein expression following ARHGAP11A knockdown. d GeneCards indicated that ARHGAP11A could directly interact with Rac1. e Rac1B expression in Hep3B cells transfected with Rac1B-HA or empty vector (NC) was analyzed with western blotting. f and g Co-IP assay using Hep3B cells transfected with Rac1B-HA determined the interaction between ARHGAP11A and Rac1B. h Blot analysis for GTP-bound RhoA, Rac1/Rac1B levels in cells, with or without ARHGAP11A knockdown following Rho GTPase pulldown experiments. Total protein levels were detected from whole cell lysate

Back to article page