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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: C3G, through its GEF activity, induces megakaryocytic differentiation and proplatelet formation

Fig. 5

Tg-C3G expression promotes CFU-MKs and increases the percentage of mature megakaryocytes in BM in vitro. a Freshly isolated BM cells were cultured with TPO for 6 days. The percentage of CD41+, CD61+ and double CD41+/CD61+ cells was analyzed by flow cytometry. Box plots represent the median ± SEM of the percentage of positive cells of 6 different measures from three independent cultures of each genotype. Mann-Whitney U test was done. *p < 0.05, **p < 0.01 and ***p < 0.001. b Table indicating the mean ± SEM of the percentage of positive cells of each genotype. c Representative images of a CFU-MK from WT-C3G and Tg-C3G (2C1 lineage) BMs. Histograms represent the mean ± SEM of the total number of CFU-MKs in BM cultures from 3 mouse of each genotype and 2 cultures per mouse. d Representative flow cytometry plots of ploidy distribution of FSChigh/CD41+ MKs (WT and Tg). We identified up to 5 different populations; 2n, 4n, 8n, 16n and ≥ 32n. The percentage of FSChigh/CD41+ MKs corresponding to each population is indicated. e Stacked bar histograms of the percentage of MKs of each genotype belonging to the different ploidy populations (2n, 4n, 8n, 16n and ≥ 32n) of BM cells. Vertical bars indicate the percentage of cells with a DNA content ≥8n. f Histograms represent the mean ± SEM of the percentage of individual ploidy populations of BM cells. Data correspond to 3 different experiments from 3 mouse of each genotype. Data were analyzed using the t-test. *p < 0.05 and **p < 0.01

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