Fig. 6
From: HMGB1/autophagy pathway mediates the atrophic effect of TGF-β1 in denervated skeletal muscle
TGF-β1 induced C2C12 myotube atrophy through HMGB1/autophagy pathway. a, b Quantification of C2C12 myotube diameter by immunofluorescence staining of MHC. Scale bar 50 μm. c Representative fluorescent images of C2C12 myotubes expressing GFP-LC3 in each group. Scale bar 100 μm. d Quantification of the average number of GFP-LC3 puncta per cell as described in (c). e Western blot analysis of LC3B, p62, MHC and HMGB1 protein expression in C2C12 myotubes. Relative grey values analyses were performed. f The effect of si-HMGB1 in C2C12 cells was evaluated by western blot. g Western blot analysis of HMGB1 in the supernatant of different groups. h ELISA analysis of HMGB1 in the supernatant of different groups. The values were obtained from three independent experiments. *P < 0.05 vs control, #P < 0.05 vs TGF-β1 group