Fig. 3
From: HMGB1/autophagy pathway mediates the atrophic effect of TGF-β1 in denervated skeletal muscle
HMGB1/autophagy pathway mediated the atrophic effect of TGF-β1 in denervated skeletal muscle. a Immunofluorescence staining of LC3B and MHC in gastrocnemius in different groups. Scale bar 50 μm. b HMGB1 staining of gastrocnemius. The arrows indicated HMGB1-positive staining. Scale bar 50 μm. c Western blot analysis of LC3B, p62, HMGB1 and MHC protein expression in gastrocnemius muscles. d Relative grey values analyses of the western blot results. 5 mice/group, *P < 0.05 vs control, #P < 0.05 vs denervation + TGF-β1 group. Den, denervation; Con, control; DAPI, 4′,6-diamidino-2-phenylindole