Fig. 1From: HMGB1/autophagy pathway mediates the atrophic effect of TGF-β1 in denervated skeletal muscleDynamic changes of TGF-β1 expression and autophagic activity during denervation-induced atrophy. a The wet weight ratio (the weight of the operational side divided by the contralateral side) of gastrocnemius and TA at indicated time points post-denervation. b Immunofluorescence staining of TGF-β1 and LC3B in gastrocnemius and TA harvested at indicated time points post-denervation. Scale bar 50 μm. c, d Western blot analysis of TGF-β1, LC3B and p62 protein expression in gastrocnemius at different time after denervation. Relative grey values analyses were performed. e, f Western blot analysis of TGF-β1, LC3B and p62 protein expression in TA at different time after denervation. Relative grey values analyses were performed. Every 5 mice were sacrificed at each time point, *P < 0.05 vs control (0 week)Back to article page