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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Adipocyte-derived IL-6 and leptin promote breast Cancer metastasis via upregulation of Lysyl Hydroxylase-2 expression

Fig. 4

Adipocyte-derived IL-6 and leptin regulate PLOD2 expression. a qRT-PCR analysis of the relative expression levels of IGF-BP1, PAI-1, IL-6, MIF, TIMP-1, TIMP-2 and Leptin in 3 T3-L1 preadipocytes, adipocytes and adipocytes cocultured with MDA-MB-231 (MB-231) breast cancer cells. Error bars represent means ± SD. **P < 0.01. b qRT-PCR analysis of relative leptin expression levels in MDA-MB-231 (MB-231) and MDA-MB-468 (MB-468) cells that were either monocultured or cocultured with adipocytes. c Dot hybridization analysis of IL-6 and leptin secretion in 3 T3-L1 preadipocytes, adipocytes and adipocytes cocultured with MDA-MB-231 (MB-231) breast cancer cells. d Dot hybridization analysis of IL-6 and leptin secretion in MDA-MB-231 (MB-231) cells and MDA-MB-468 (MB-468) cells cocultured with adipocytes. e MDA-MB-231 (MB-231) and MDA-MB-468 (MB-468) breast cancer cells were monocultured or cocultured with adipocytes. After 3 days, tumor cells were collected and GP130 and PLOD2 protein expression was examined by Western blotting. f MDA-MB-231 (MB-231) and MDA-MB-468 (MB-468) breast cancer cells were monocultured or cocultured with adipocytes. After 3 days, tumor cells were collected and OBR and PLOD2 protein expression was examined by Western blotting. g MDA-MB-231 (MB-231) breast cancer cells were monocultured or cocultured with adipocytes. A blocking antibody directed against IL-6 was added to the culture medium of experimental cells. After 3 days, tumor cells were harvested to detect PLOD2 protein expression. h PLOD2 protein expression was analyzed by Western blot in MDA-MB-231 (MB-231) and MDA-MB-468 (MB-468) breast cancer cells transiently transfected with either vector or one of two siRNAs against the leptin receptor (OBR) and then either monocultured or cocultured with adipocytes

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