Fig. 1

PLOD2 is upregulated during adipocyte-driven migration and invasion in breast cancer cells. a Schematic diagram of the coculture system. Adipocytes were seeded in the bottom layer and breast cancer cells were seeded in the upper layer of the Transwell culture system. Cells were then cocultured for 3 days. b Mature adipocytes were stained with Oil Red O to confirm differentiation efficiency. c-d MDA-MB-231 (MB-231) or MDA-MB-468 (MB-468) cells were cocultured with adipocytes (Co) or monocultured (Control) for 3 days. Then, cancer cells were used for migration (c) and 3D matrix invasion assays (d). Error bars represent means ± SD. ^**P < 0.01. Scale bars, 100 μm. e Relative mRNA expression levels of P4HA1, PLOD1, PLOD2, PLOD3, LOX and Col1A1 in MDA-MB-231 breast cancer cells cocultured with adipocytes or monocultured for 3 days. Error bars represent means ± SD. ^**P < 0.01. f MDA-MB-231 (MB-231), MDA-MB-468 (MB-468) breast cancer cells were cocultured with adipocytes or monocultured. After 3 days, cells were harvested and PLOD2 protein expression was detected by Western blotting. MDA-MB-231 (MB-231) cancer cells were cocultured with human adipocytes. After 3 days, cells were harvested and PLOD2 protein expression was detected by Western blotting