Fig. 4

Tyro3 and Axl, but not Mertk, are required for platelet accumulation in vivo. Cremaster arteriole injury was induced in Tyro3^−/−, Axl^−/−, Mertk^−/−, and wild-type mice. Platelets were detected using anti-CD41 F(ab)2 fragments (binding to platelet αIIb) conjugated with Alexa Fluor 488. (a) Using intravital microscopy, representative fluorescence images show platelet accumulation (green) at selected time points up to 180 s after vascular injury. The median integrated fluorescence intensities (FI) of anti-CD41 antibody over 270 s (platelet, b). The data were analyzed by the area under the curve (AUC) with one-way ANOVA and Kruskal-Wallis test. The data were obtained from 30 thrombi in 3 mice for each experimental condition. NS = not significant, *** P < 0.001