Fig. 2

The decreased JON/A binding and P-selectin expression in Tyro3^−/−, Axl^−/− but not Mertk^−/− platelets. Washed platelets from wild type, Tyro3^−/−, Axl^−/− or Mertk^−/− mice were stimulated with 0.4 μg mL^− 1 CRP or 0.015 U mL^− 1 thrombin, followed by incubation with PE-labeled JON/A antibody (a, b) and PE-labeled anti-P-selectin antibody (c, d). The samples were analyzed using flow cytometry. MFI = mean fluorescent intensity. Mean ± SEM, NS = not significant, *** P < 0.001. One-way ANOVA and Dunnett’s multiple comparison test