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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling

Fig. 4

Cellular localization of components of MAPK pathway in undifferentiated hiPSCs. a Simple nucleocytoplasmic fractionation. hiPSCs were fractionated into three distinct fractions (TCL, total cell lysate; Cyt, cytosolic fraction; Nuc, nuclear fraction) by using rapid non-ionic detergent-based purification technique. b Immunoblot analysis of components of MAPK pathway showed the dominant localization of all proteins in the cytosolic fraction in undifferentiated hiPSCs, before and after bFGF stimulation. c Immunoblot analysis of p-RSK in undifferentiated (CM-100) and differentiated hiPSCs (CM-0 and non-CM). Total amounts of RSK and γ-tubulin served as loading controls. d The graph represents densitometric analysis of three independent experiments, each carried out in duplicates. All values were normalized to γ-tubulin and relative to CM-100. Data are shown as mean ± SD

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