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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

Fig. 5

Overexpression of jumu induces cell spreading and large numbers of filopodia in hemocytes. a-d Phalloidin staining (green) shows that Hml > GFP > UAS-jumu displays an increased number of filopodia and larger lamellipodia than the control (a and b); knockdown of ena or fascin in Hml > GFP > UAS-jumu can inhibit cell spreading and the formation of numerous filopodia (c and d). e-g Quantification of filopodium numbers and lengths and lamellipodial area. h Quantification of the phagocytosis indexes for latex beads, B. bassiana, S. aureus or E. coli. i-l Quantification of Ena, Fascin, Rho1 and Profilin levels. m Western blot analysis using antibodies against Ena, Fascin, Profilin and Rho1 and the corresponding graphical representation show that the levels of Ena and Fascin are increased in jumu-overexpressing S2 cells compared with the expression in the controls, whereas the levels of Profilin are reduced and the levels of Rho1 are unchanged. Error bars represent the S.E.M of at least 3 independent experiments; NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test). Scale bars: 10 μm

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