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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-κB pathway in breast cancer cells

Fig. 4

CDKN1A is transcriptionally regulated by Dox and affects the expression of PTN in breast cancer cells. a MDA-MB-231 cells were treated with Dox (1 μg/ml) or PBS for 24 h, and a heatmap of the 20 genes that showed the largest changes in gene expression between the two groups determined by microarray analysis is shown. b The correlation analyses of PTN and CDKN1A expression in breast cancer specimens with or without chemotherapy from the GEO database are shown, n = 69, Pearson’s r test. c The paired expression data of CDKN1A in breast cancer specimens before and after adjuvant chemotherapy were obtained from the GEO database, n = 69, t-test, ***p < 0.001. d, e, f Immunoblot analyses of the expression of CDKN1A, PTN, and PTPRZ1 in MDA-MB-231 cells undergoing treatment with Dox and knockdown or increasing the expression of CDKN1A by siRNA treatment or plasmid overexpression, respectively, are shown. Representative blots are shown in the upper panel, and summary densitometry measurements are shown in the lower panel. The data are shown as the mean ± s.e.m., n = 5, *p < 0.05, n.s. means not significant, Student’s t-test

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