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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-κB pathway in breast cancer cells

Fig. 3

PTN/PTPRZ1 are involved in breast cancer cell chemoresistance. MDA-MB-231 cells, which were transfected with siPTPRZ1, PTPRZ1, or siPTN to regulate endogenous protein, or added exogenous protein (rhPTN) in the cell culture medium to increase PTN levels, were treated with different concentrations of Dox or DDP. a, b Cell viability was determined with a CCK8 assay. c Cell growth was measured by colony formation assays. The data are shown as the mean ± s.e.m. of three independent experiments. The statistical significance of the between-group differences was evaluated with a Student’s t-test, *p < 0.05, **p < 0.01, n.s. means not significant. d Apoptosis was measured by flow cytometry based on Annexin V/PI double staining. The data are shown as the mean ± s.e.m. of three independent experiments. The statistical significance of the between-group differences was evaluated with a Student’s t-test, *p < 0.05, **p < 0.01

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