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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes

Fig. 6

Stereotaxic injection of lenti-pre-AMO-1 into the hippocampi of Tg mice improved SV distribution. a Stereotaxic injection of lenti-pre-AMO-1 into the CA1 domain reduced the miR-1 level in the hippocampi of Tg mice as determined by qRT-PCR analysis. PLevene = 0.01, one-way ANOVA: F = 10.872, P = 0.01; Fisher’s PLSD test: PWT:Tg = 0.005, PTg + lenti-pre-mis-AMO-1::Tg + lenti-pre-AMO-1 = 0.012. n = 3 batches of cells for each group. b Stereotaxic injection of lenti-pre-AMO-1 into the hippocampi of Tg mice derepressed the expression of SNAP-25 protein. PLevene = 0.004, one-way ANOVA: F = 15.700, P < 0.001; Fisher’s PLSD test: PWT:Tg = 0.001, PTg + lenti-pre-mis-AMO-1:Tg + lenti-pre-AMO-1 < 0.0001. n = 6 animals for each group. c Representative electron micrographs showing the effect of stereotaxic injection of lenti-pre-AMO-1 on SVs distribution at presynaptic active zone of Tg mice. d Lenti-pre-AMO-1 prevented an increase in the total number of SVs in the hippocampal synapses of Tg mice. PLevene < 0.003, one-way ANOVA: F = 33.161, P < 0.0001; Fisher’s PLSD test: PWT:Tg < 0.0001, PTg + lenti-pre-mis-AMO-1:Tg + lenti-pre-AMO-1 < 0.0001. n = 20 active zones from 3 mice for each group. e Lenti-pre-AMO-1 redistributed SV location in Tg mice nerve terminals. 0 nm shell: PLevene < 0.012, one-way ANOVA: F = 2.928, P = 0.062; Fisher’s PLSD test: PWT:Tg = 0.596, PTg + lenti-pre-mis-AMO-1: Tg + lenti-pre-AMO-1 = 0.024; 50 nm shell: PLevene = 0.044, one-way ANOVA: F = 7.517, P = 0.01; Fisher’s PLSD test: PWT:Tg = 0.003, PTg + lenti-pre-mis-AMO-1:Tg + lenti-pre-AMO-1 = 0.001; 100 nm shell: PLevene = 0.055, one-way ANOVA: F = 11.161, P < 0.0001; Fisher’s PLSD test: PWT:Tg = 0.014, PTg + lenti-pre-mis-AMO-1:Tg + lenti-pre-AMO-1 < 0.0001. n = 20 synapses s from 3 mice for each group. f Lenti-pre-AMO-1 did not affect the length of active zone. PLevene = 0.551, one-way ANOVA: F = 0.404, P = 0.670; Fisher’s PLSD test: PWT:Tg = 0.940, PTg + lenti-pre-mis-AMO-1:Tg + lenti-pre-AMO-1 = 0.419. n = 20 active zones from 3 mice for each group. *P < 0.05 versus WT; #P < 0.05 versus Tg

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