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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes

Fig. 5

MiR-1 influences vesicle exocytosis by inhibiting the expression of SNAP-25 directly. a MiR-1 levels in NRNs transfected with miR-1 and Snap25-ODN. PLevene = 0.041, one-way ANOVA: F = 17.566, P = 0.001; Fisher’s PLSD test: Pmis-miR-1: miR-1 = 0.01, Pmis-miR-1: Snap25-ODN = 0.001. n = 3 batches of cells for each group. b Derepression of SNAP-25 by Snap25-ODN in NRNs co-transfected with miR-1. PLevene = 0.515, one-way ANOVA: F = 18.101, P = 0.001; Fisher’s PLSD test: Pmis-miR-1: miR-1 = 0.01, Pmis-miR-1: Snap25-ODN < 0.0001. *P < 0.05 versus mis-miR-1-control vector; #P < 0.05, versus miR-1. n = 3 batches of cells for each group. c The FM1–43 dye fluorescence change of NRNs co-transfected with miR-1 and Snap25-ODNs. d Snap25-ODN failed to improve the decline in FM1–43 signalling (F/F0) in NRNs transfected with miR-1 alone. χ2Mauchly = 1935.529, P < 0.0001; Ftotal (19, 1407) = 35.946, P < 0.0001; Fisher’s PLSD test: Pmis-miR-1: miR-1 < 0.0001, PmiR-1: miR-1 + Snap25-ODN < 0.0001, Pmis-miR-1: miR-1 + Snap25-ODN = 0.730. n = 25 neurons from 3 batches of cells for each group

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