Fig. 3

Expression and Epigenomic status of miR-96, miR-182, and AMAP1. a Expression levels of miR-96, miR-182, and AMAP1 in MDA-MB-231 cells, MCF7 cells, and BJ cells were measured by quantitative RT-PCR. The unpaired t-test was performed (miR-96: n = 2; miR-182, AMAP1: n = 3) and the results are shown as the mean ± SEM. **P < 0.01. #: The mRNA level of BJ cell was under the detection limit. b Expression levels of AMAP1 were assessed by immunoblotting. c The ENCODE data of miR-183-96-182 and miR-423 loci by UCSC Genome Browser are shown. Definitions of the colors are given at the top. hESC: human embryonic stem cells, HMEC: human mammary epithelial cells, NHEK: normal human epidermal keratinocytes, NHLF: normal human lung fibroblasts, GM12878: B-lymphocyte transformed by Epstein-Barr virus, K562: highly undifferentiated and granurocytic myelogenous leukemia cells, HepG2:hephepatocytellular carcinoma cells, HUVEC: human umbilical vein endothelial cells, HSMM: human skeletal muscle myoblasts