Fig. 2

miR-96 and miR-182 target AMAP1 mRNA. a Microarray analysis of gene expression in MDA-MB-231 cells (parental) and their p53 derivatives. Green, black, and red indicate transcript levels below, equal to, or above the mean, respectively, as shown at the bottom, in which gene expression intensities are represented in the log 2 scale. b Thirty-two miRNAs were expressed at higher levels in shp53/wt cells than in parental and shp53 cells (a), and 9 of them had homology to the 3’-UTR of the AMAP1 mRNA. c Correlation diagrams between expression levels of miRNA candidates and AMAP1 mRNA are shown. d The interactions among the AMAP1 mRNA 3’-UTR (wt), or its mutant (mt) and miR-96, miR-182, or miR-301a, were assessed by luciferase reporter assay in HEK293T cells. Luciferase activities were measured and normalized to Renilla luciferase activity. The results are shown as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01. e mRNA expression levels of two miRNA candidates in MDA-MB-231 cells and their p53 derivatives, and HMLE cells and their p53-knockdown cells were measured by quantitative RT-PCR. The results are shown as the mean ± SEM (n = 2). n.s. means no significance. *P < 0.05