Fig. 7
IMPACT overexpression does not appear to modulate Integrated Stress Response in tryptophan-deprived GL261 cells. a) A representative Western blot assessing the ISR protein content in GL261-IMPACThigh (High) and GL261 wild-type (WT) cells cultured for 24 h in 50 μM or 5 μM tryptophan media. b) Normalised band intensity of phosphorylated eIF2α, ATF4 and CHOP pooled from three independent repeats of the experiment in panel A. Bar heights and whiskers represent mean ± SD